بررسی میزان فعالیت آنزیم های ماتریکس متالوپروتئیناز 8 و میلوپراکسیداز بزاق در افراد سیگاری و غیر سیگاری

Introduction & Objectives: Smoking appears to be one of the concerns and priorities of public health in modern and developing societies in the world. However, no study has been conducted so far on the damages caused by cigarette smoke on the activities of matrix metalloproteinase 8 and myeloperoxidase enzymes in the saliva in smokers and non-smokers. Thus, the main objective of this study was to evaluate the effect of smoking on the functions of matrix metalloproteinase 8 and myeloperoxidase enzymes of saliva. Materials & Methods: This research was a case - control study. The study population consisted of 34 smokers and 34 non-smokers who were selected from those in the age range from 20 to 50 years that had referred to the School of Dentistry of Tabriz who had no history of using immunosuppressive drugs in the last two years or any chronic systemic diseases such as diabetes, cardiovascular diseases, etc. The saliva samples were collected using the spitting method. The expression rate of salivary matrix metalloproteinase-8 and myeloperoxidase enzymes were measured by Real Time PCR methods. The salivary myeloperoxidase and matrix metalloproteinase-8 enzymes activity were detection by ELISA method. Results: The mean activity rate of myeloperoxidase enzyme in the case and control groups was equal to 0.52 (IU/mgProtein) and 0.26 (IU/mgProtein), respectively, with a P-value less than 0.0001. The mean gene expression rate of myeloperoxidase in the case and control groups was equal to 1.25 (CT) and 0.82 (CT), respectively, with a P-value less than 0.0001. The mean activity rate of matrix metalloproteinase 8 enzyme in the case (smokers) and control (non-smokers) groups was equal to 331.75 (IU/mgProtein) and 128.53 (IU/mgProtein), respectively, with a P-value less than 0.0001. Discussion: The results of this study showed that smoking led to significant increases in both gene expression rate and myeloperoxidase enzyme activity rate in smokers than the non-smokers. In addition, the gene expression rate and activity of matrix metalloproteinase -8 enzyme in the saliva of smokers showed a significant increase compared to the control group. Keywords: Matrix metalloproteinase 8, Myeloperoxidase, Smoking, Saliva